Journal: Stem Cells Translational Medicine

Article Title: Extracellular Vesicles From Mesenchymal Umbilical Cord Cells Exert Protection Against Oxidative Stress and Fibrosis in a Rat Model of Bronchopulmonary Dysplasia

doi: 10.1093/stcltm/szad070

Figure Lengend Snippet: Antibody list.

Article Snippet: Goat anti RAGE , AF1145 , BIO-TECHNE.

Techniques:

Journal: Cardiovascular Diabetology

Article Title: Circulating levels of AGEs and soluble RAGE isoforms are associated with all-cause mortality and development of cardiovascular complications in type 2 diabetes: a retrospective cohort study

doi: 10.1186/s12933-022-01535-3

Figure Lengend Snippet: Comparison of AGEs, sRAGE isoforms, and their ratios between healthy controls (CTR) and patients with type 2 diabetes

Article Snippet: Specifically, total human sRAGE included the detection of both cRAGE and esRAGE variants (DY1145, Human RAGE DuoSet ELISA, R&D Systems Inc., MN, USA) and esRAGE concentration was evaluated by an ELISA assay with an antibody raised against the exclusive C-terminal amino acids (332–347) sequence (K1009-1, B-bridge International, CA, USA). cRAGE was determined by subtracting esRAGE from sRAGE as already described [ , , ].

Techniques: Comparison

Journal: Cardiovascular Diabetology

Article Title: Circulating levels of AGEs and soluble RAGE isoforms are associated with all-cause mortality and development of cardiovascular complications in type 2 diabetes: a retrospective cohort study

doi: 10.1186/s12933-022-01535-3

Figure Lengend Snippet: Boxplots for the comparison of A total sRAGE, esRAGE, and cRAGE isoforms and ) AGEs/sRAGE, AGEs/esRAGE, AGEs/cRAGE, cRAGE/esRAGE ratio among healthy control subjects (CTR) and type 2 diabetes patients without (T2DM-NC) or with (T2DM-C) complications. **p < 0.01; ***p < 0.001; ****p < 0.0001 for Dunn’s post-hoc tests following Kruskal-Wallis H test

Article Snippet: Specifically, total human sRAGE included the detection of both cRAGE and esRAGE variants (DY1145, Human RAGE DuoSet ELISA, R&D Systems Inc., MN, USA) and esRAGE concentration was evaluated by an ELISA assay with an antibody raised against the exclusive C-terminal amino acids (332–347) sequence (K1009-1, B-bridge International, CA, USA). cRAGE was determined by subtracting esRAGE from sRAGE as already described [ , , ].

Techniques: Comparison, Control

Journal: Cardiovascular Diabetology

Article Title: Circulating levels of AGEs and soluble RAGE isoforms are associated with all-cause mortality and development of cardiovascular complications in type 2 diabetes: a retrospective cohort study

doi: 10.1186/s12933-022-01535-3

Figure Lengend Snippet: Spearman correlations of AGEs and the different isoforms of sRAGE isoforms with age in healthy controls (CTR) and patients with type 2 diabetes

Article Snippet: Specifically, total human sRAGE included the detection of both cRAGE and esRAGE variants (DY1145, Human RAGE DuoSet ELISA, R&D Systems Inc., MN, USA) and esRAGE concentration was evaluated by an ELISA assay with an antibody raised against the exclusive C-terminal amino acids (332–347) sequence (K1009-1, B-bridge International, CA, USA). cRAGE was determined by subtracting esRAGE from sRAGE as already described [ , , ].

Techniques:

Journal: Cardiovascular Diabetology

Article Title: Circulating levels of AGEs and soluble RAGE isoforms are associated with all-cause mortality and development of cardiovascular complications in type 2 diabetes: a retrospective cohort study

doi: 10.1186/s12933-022-01535-3

Figure Lengend Snippet: Age- and HbA1c-adjusted multiple quantile regression model for the evaluation of AGEs and sRAGE isoforms in type 2 diabetes complications

Article Snippet: Specifically, total human sRAGE included the detection of both cRAGE and esRAGE variants (DY1145, Human RAGE DuoSet ELISA, R&D Systems Inc., MN, USA) and esRAGE concentration was evaluated by an ELISA assay with an antibody raised against the exclusive C-terminal amino acids (332–347) sequence (K1009-1, B-bridge International, CA, USA). cRAGE was determined by subtracting esRAGE from sRAGE as already described [ , , ].

Techniques:

Journal: Cardiovascular Diabetology

Article Title: Circulating levels of AGEs and soluble RAGE isoforms are associated with all-cause mortality and development of cardiovascular complications in type 2 diabetes: a retrospective cohort study

doi: 10.1186/s12933-022-01535-3

Figure Lengend Snippet: Univariate and multivariate Cox regression analysis for the prediction of 15-year all-cause mortality in patients with type 2 diabetes

Article Snippet: Specifically, total human sRAGE included the detection of both cRAGE and esRAGE variants (DY1145, Human RAGE DuoSet ELISA, R&D Systems Inc., MN, USA) and esRAGE concentration was evaluated by an ELISA assay with an antibody raised against the exclusive C-terminal amino acids (332–347) sequence (K1009-1, B-bridge International, CA, USA). cRAGE was determined by subtracting esRAGE from sRAGE as already described [ , , ].

Techniques:

Journal: Respiratory Research

Article Title: Serum AGE/RAGEs as potential biomarker in idiopathic pulmonary fibrosis

doi: 10.1186/s12931-018-0924-7

Figure Lengend Snippet: ELISA of AGEs and sRAGE in serum blood from controls (CNT) and fibrosing ILDs. a and b ELISA for total AGEs and sRAGE showed significant differences between control group with IPF and cHP (* p < 0.01), and differences between fNSIP patients with IPF (# p < 0.01) and cHP (§ p < 0.01) groups. However, there were no significant differences between IPF with cHP and control with fNSIP. c AGEs/sRAGE ratio estimated by ELISA was greater in IPF and cHP groups compared with control (* p < 0.01). The ratio also allowed to distinguish between IPF patients with cHP and fNSIP (# p < 0.05), and cHP with fNSIP group (§ p < 0.01), whereas this did not distinguish between control and fNSIP patients. Data were analyzed by one-way ANOVA following by the appropriate post-hoc analysis

Article Snippet: AGE and sRAGE were measured by specific commercial ELISA kit; Human RAGE Quantikine ELISA Kit (DRG00; R&D Systems, USA) and Human AGEs ELISA Kit (CSB-E09412h; Cusabio Biotech Co., China), following the manufacturer’s recommendations.

Techniques: Enzyme-linked Immunosorbent Assay, Control

Journal: Respiratory Research

Article Title: Serum AGE/RAGEs as potential biomarker in idiopathic pulmonary fibrosis

doi: 10.1186/s12931-018-0924-7

Figure Lengend Snippet: ELISA measurements and lung function from IPF patients at 3-year interval

Article Snippet: AGE and sRAGE were measured by specific commercial ELISA kit; Human RAGE Quantikine ELISA Kit (DRG00; R&D Systems, USA) and Human AGEs ELISA Kit (CSB-E09412h; Cusabio Biotech Co., China), following the manufacturer’s recommendations.

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Respiratory Research

Article Title: Serum AGE/RAGEs as potential biomarker in idiopathic pulmonary fibrosis

doi: 10.1186/s12931-018-0924-7

Figure Lengend Snippet: Correlation between changes in ELISA measurements and lung function

Article Snippet: AGE and sRAGE were measured by specific commercial ELISA kit; Human RAGE Quantikine ELISA Kit (DRG00; R&D Systems, USA) and Human AGEs ELISA Kit (CSB-E09412h; Cusabio Biotech Co., China), following the manufacturer’s recommendations.

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Respiratory Research

Article Title: Serum AGE/RAGEs as potential biomarker in idiopathic pulmonary fibrosis

doi: 10.1186/s12931-018-0924-7

Figure Lengend Snippet: Correlation between changes in DLCO and differences in sRAGE-AGEs levels in IPF patients. a In IPF patients, a decline of DLCO was related with a decrease of sRAGE in serum ( r = 0.555, p < 0.01). b and c On the contrary, AGEs levels and the AGEs/sRAGE ratio were negatively correlated with the percentage of changes in DLCO in IPF patients ( r = − 0.747 and − 0704, respectively, p < 0.01), meaning poor lung function in patients with an increase of AGEs-sRAGE imbalance over time. The changes in serum sRAGE and AGEs and lung function from IPF patients was calculated, adjusting over time: Δ ELISA in a month = [ELISA final – ELISA initial]/month; change of % predicted PFT = [PFT final – PFTs initial]/month. Pearson’s correlation coefficients were performed to analyze the lineal relationship between the variables

Article Snippet: AGE and sRAGE were measured by specific commercial ELISA kit; Human RAGE Quantikine ELISA Kit (DRG00; R&D Systems, USA) and Human AGEs ELISA Kit (CSB-E09412h; Cusabio Biotech Co., China), following the manufacturer’s recommendations.

Techniques: Enzyme-linked Immunosorbent Assay